摘要
在单因素实验的基础上,采用Box-Behnken实验设计方法,以漆酶酶活为响应值,对杏鲍菇发酵产漆酶培养条件进行优化,并经实验验证模型的可行性。最终得到优化后的产酶条件为:转速131r/min,装液量32mL,温度27.6℃;在此条件下杏鲍菇对菲具有较高的降解率,培养12d后,菲降解率达82.86%。
Based on the single factor experiment,using Box-Behnken experimental design method,the enzyme activity of laccase as response value ,the fermentation conditions were optimized and the feasibility of experiment model was validated. The results indicated optimal ingredients including ：the speed at 131r/min,temperature at 27.6℃ and shake loading at 32mL/100mL. Under the optimal conditions,the strain of Pleurotus eryngii had higher degradation rate of phenanthrene. The degradation rate of phenanthrene reached 82.86% at the 12th day.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第14期221-225,共5页
Science and Technology of Food Industry
基金
安徽省高等学校优秀青年人才基金项目(2011SQRL079)
安徽高校省级自然科学研究项目(KJ2013A049)
关键词
杏鲍菇
漆酶
培养条件
响应面法
菲
Pleurotus eryngii
laccase
culture conditions
response surface methodology
phenanthrene
