介孔氧化铝净化-气相色谱法同时测定蔬菜、水果及茶叶中16种有机磷农药残留

Simultaneous determination of 16 organophosphorous pesticides in vegetables,fruits and tea by gas chromatography coupled with clean-upbymesoporous alumina as solid-phase extraction adsorbent

利用气相色谱检测技术,建立了能应用于白菜、生菜、南瓜、洋葱、番茄、白萝卜、苹果、梨及茶叶等不同基质中16种有机磷农药残留的检测方法。样品采用乙酸乙酯提取,经以合成的介孔氧化铝作吸附剂的固相萃取柱净化,DB-1701毛细管柱分离,气相色谱-火焰光度检测器检测。结果表明,蔬菜、水果及茶叶基质中16种有机磷农药在10-2 000μg/L范围内具有良好的线性关系（R^2〉0.997）;平均回收率为83.2%-103.8%,相对标准偏差为2.0%-9.9%。该方法灵敏度高、准确度高、重复性好,可适用于蔬菜、水果及茶叶基质中16种有机磷残留量的同时测定。

A gas chromatographic method based on solid-phase extraction was developed for the simultaneous determination of 16 organophosphorous pesticides in vegetables,fruits and tea,including cabbage,lettuce,pumpkin,onion,tomato,turnip,apple,pear and tea.The samples were extracted with ethyl acetate,and clean-up with mesoporous alumina as solid-phase extraction adsorbent.The separation of target compounds was performed on a DB-1701 capillary column,and the quantitative analysis of the organophosphorous pesticides was carried out by gas chromatography with flame photometric detection.The results showed that the calibration curves of the 16 organophosphorous pesticides were linear in the range of 10-2 000μg/L with good correlation coefficients（R^2〉0.997）.The recoveries of the pesticides in different samples at three spiked levels ranged from83.2%to 103.8% with the relative standard deviations of 2.0%-9.9%.This method has high sensitivity,high accuracy and good repeatability,and can be applied to the determination of the organophosphorus pesticide residues in vegetables,fruits and tea.