用于半胱氨酸检测的比率荧光探针的合成与应用

Synthesis and Application of the Ratiometric Fluorescent Sensor for Cysteine

合成了一个衍生于吡咯并吡咯二酮的用于半胱氨酸（Cys）检测的比率荧光探针，用核磁共振谱和质谱鉴定了其结构。探针与Cys作用后荧光发射最大波长由576 nm蓝移至536 nm，荧光颜色由黄色变为绿色，可裸眼直观识别。并且该探针可以专一性地识别Cys而不受其他氨基酸的影响。当Cys溶液浓度在0～300 μmol/L内变化时，该探针荧光变化F536/F576符合线性关系（R = 0.997），可以实现其定量检测。

A ratiometric fluorescent sensor for Cysteine （Cys） was synthesized based on Diketopyrrolopyrrole and then characterized by 1H-NMR and MS. The maximum emission wavelength located at 576 nm of the sensor displayed a blue-shift to 536 nm induced by Cys and meanwhile, the fluorescence color changed from yellow to green which could be observed through naked eyes. Moreover, DPP-CHO was experimentally proved a specific sensor to Cys without interferences of other amino acids. Whereafter, its utility was carried out to detect Cys quantitatively according to the liner relationship between concentrations of Cys （0～300 μmol/L） and relevant F536/F576 values of the sensor.