超高效液相色谱-串联质谱检测蔬果中多杀霉素的残留

Determination of Spinosad Residues in Vegetables and Fruits by UPLC-MS/MS

【目的】建立超高效液相色谱-串联质谱（UPLC—MS／MS）检测蔬果中残留分析方法。[方法]采用乙腈提取多杀霉素A、D，UPLC—MS／MS进行定性和定量。【结果】多杀霉素A、D在0．005-0．5mg／L范围内线性关系良好，相关系数均在0．9995以上；在0．02~0．5mg／L添加质量浓度范围内，回收率为72.3％-116.3％，相对标准偏差为1．10％-4．92％；最低检出质量浓度为0．5μg／L。[结论]方法简便、快速、定量准确、灵敏度和精密度好，可实现蔬菜水果中多杀霉素农残分析的要求。

[Aims] An ultra-performance liquid chromatography tandem mass spectrometry （UPLC-MS/MS） analytical method was established for the determination of spinosad residues in the vegetables and fruits. [Methods] Samples were extracted by acetonitrile, detected by UPLC-MS/MS. [Results] The linear ranges of spinosyn A and D were 0.005-0.5 mg/L, and the correlation coefficients were all above 0.999 5. The average recoveries of spinosyn A and D in samples ranged from 72.3 to 116.3% at the levels of 0.02-0.5 mg/L, and the relative standard deviations were between 1.10 to 4.92%, the limit of quantification was 0.5 μg/L. [Conclusions] The method is simple, rapid, accurate and sensitive for the determination of spinosyn A and D residues in vegetables and fruits.