反相高效液相色谱法测定瘤胃内容物中挥发性脂肪酸的方法研究

Study on the determination of volatile fatty acids in rumen fluid by reversed phase high performance liquid chromatography

本实验以山羊瘤胃液为样品,建立了反相高效液相色谱(RP-HPLC)测定乙酸、丙酸和丁酸等挥发性脂肪酸含量的方法。结果表明:甲醇与0.01 mol/L磷酸二氢钠缓冲液(pH=2.6)比例为28∶72时可很好地分离乙酸、丙酸和丁酸,回收率均大于83%,相对标准偏差(RSD)均小于0.67%。

Goat rumen fluid was used to establish a method of determination acetic,propionic and butyric acid by using reversed phase high performance liquid chromatography（RP-HPLC）.The results showed that：acetic,propionic and butyric acid could be well separated by the methanol mixed with sodium dihydrogen phosphate buffer （0.01 mol/L pH=2.6） by 28∶72,and the recoveries of the three volatile fatty acids （VFA）were larger than 83%,relative standard deviation （RSD） were less than 0.67%.