血红素加氧酶-1对人宫颈癌细胞株Hela增殖及迁移能力的影响

EFFECTS OF HEME OXYGENASE-1 ON THE PROLIFERATION AND INVASION ABILITY OF HUMAN CERVICAL CARCINOMA HELA CELL LINE

目的观察血红素加氧酶-1(Heme Oxygenase-1,HO-1)对人宫颈癌细胞株Hela增殖及迁移能力的影响。方法培养人宫颈癌细胞株Hela,以MTT比色法及平板克隆形成实验观察HO-1对Hela细胞增殖的作用;Transwell迁移实验检测Hela细胞迁移能力。Western blot测定p-p38、MMP-2表达。结果 MTT实验、克隆形成实验及Transwell迁移实验显示转染HO-1后Hela细胞增殖及侵袭能力均明显下调。Western blot结果显示HO-1表达升高可抑制p-p38、MMP-2表达。结论 HO-1可能通过抑制p-p38表达,抑制人宫颈癌细胞株Hela增殖;并通过下调MMP-2表达抑制其侵袭能力。

Objective To observe the effects of Heine Oxygenase - 1 on the proliferation and invasion ability of human cervical carcinoma Hela cell line in vitro. Methods The effects of HO - 1 on cell proliferation was observed by MrFF and plate clone formation assay ;human cervical carcinoma Hela cell line invasion ability was observed by Transwell assays;p-p38 and MMP -2 expressions were assessed by Western blot. Results HO -1 could depress the proliferation and invasion ability of Hela cell. p -p38 and MMP -2 expressions were depressed by HO - 1. Conclusion HO - 1 can depress the proliferation and invasion of Hela cell. The anti - proliferation effect of HO - 1 may be due to regulating p- p38 and MMP- 2 expressions.