藏药帕珠丸对慢性酒精性肝损伤大鼠肝组织IL-1、IL-6、TNF-α、TGF-β_1含量及TGF-β_1 mRNA表达的影响

INFLUENCE OF PAZHUWAN ON THE LEVELS OF IL-1,IL-6,TNF-α,TGF-β_1 AND THE TGF-β_1 mRNA EXPRESSION IN LIVER WITH ALCOHOLIC LIVER DISEASE

目的观察藏药帕珠丸对酒精性肝损伤大鼠肝组织IL-1、IL-6、TNF-α、TGF-β1含量及TGF-β1mRNA表达的影响。方法分别给予慢性酒精性肝损伤模型大鼠50、100、200 mg·kg-1帕珠丸混悬液,观察其对肝组织IL-1、IL-6、TNF-α、TGF-β1含量及TGF-β1mRNA表达的影响。结果藏药帕珠丸低、中、高剂量组肝组织IL-1、IL-6、TNF-α、TGF-β1含量均显著降低(P<0.05),TGF-β1mRNA表达下降(P<0.05)。结论藏药帕珠丸可能是通过降低肝组织中IL-1、IL-6、TNF-α、TGF-β1含量及TGF-β1mRNA表达,对大鼠慢性酒精性肝损伤发挥保肝作用。

Objective To observe the effects of Pazhuwan on the levels of IL - 1, IL -6, TNF - α, TGF - β1 and the mRNA expression of TGF - β1 in Rat hepatic tissue with alcoholic liver disease （ALD）. Methods ALD model was established by feeding SD male rats with alcoholic,Pazhuwan （50,100, 200 mg · kg^-1） was orally administrated to experiment groups of rats. The IL- 1, IL- 6 ,TNF- α, TGF- β1 levels and the mRNA expression of TGF - β1 in hepatic tissue were tested. Results The levels of IL - 1, IL - 6, TNF - α, TGF - β1 in the Pazhuwan group were significantly decreased （ P 〈 0. 05 ）. The same changes were observed in the mRNA expression of TGF -β1 in hepatic tissue. Conclusion Pazhuwan is likely to protecting the chronic alcoholic injury liver by inhibition inflammation cytokines levels.