摘要
目的探讨姜黄素诱导人骨肉瘤细胞株MG63凋亡的机制。方法不同浓度(0、25、50和75μM)姜黄素作用MG63细胞24 h后,CCK8法检测细胞体外增殖,TUNEL染色法检测细胞凋亡,Western blotting检测AKT/Bax-Bcl-2/Caspase 3等内源性凋亡途径相关蛋白表达水平。结果 CCK8实验结果显示在0~75μM范围内,50μM以上浓度的姜黄素能显著抑制MG63增殖,诱导细胞凋亡、p-AKT蛋白水平明显降低,而Bax蛋白及剪切的Caspase 3蛋白水平则显著增高。结论姜黄素通过影响AKT/BaxBcl-2/Caspase 3途径诱导MG63细胞凋亡。
Objective To study the effect of curcumin on the apoptosis of osteosarcoma cell line MG63.Methods After MG63 cells were treated with different concentration(0、25、50 and 75 μM) curcumin for 24 h,CCK8 assay was used to evaluate the proliferation and TUNEL assay was used to investigate the apoptosis of MG63 cells.Western blotting was performed to detect the expression level of p-AKT,Bax,Bcl-2 and Caspase 3.Results During the concentration of 0 to 75 μM,curcumin above 50 μM could inhibit the proliferation,induce the apoptosis of MG63 cells,and could inhibit the phosphorylation of AKT protein,while upregulate the expression of Bax and cleaved 3.Conclusion Curcumin could induce MG63 cells apoptosis by regulating AKT/Bax-Bcl-2/Caspase 3 pathway.
出处
《现代中药研究与实践》
CAS
2014年第3期36-38,共3页
Research and Practice on Chinese Medicines
基金
国家自然科学基金青年项目(No.81302357
81300085)
广东省自然科学基金博士启动项目(No.S2013040016493
S2013040013895)
广东高校优秀青年创新人才培养计划项目育苗工程[自然科学](No.2013LYM_0075)