VEGF及其受体sFlt-1在子痫前期患者血清中的变化及意义

Changes and significance of serum VEGF and sFlt-1 in preeclampsia pregnant women

目的探讨子痫前期患者血清中血管内皮生长因子(VEGF)及其可溶性血管内皮生长因子受体1(sFlt-1)的水平及意义。方法选择2011年8月至2012年3月子痫前期患者共60例(研究组),其中轻度子痫前期孕妇30例,重度子痫前期孕妇30例,以同期正常晚期妊娠孕妇30例作为对照组。采用酶联免疫吸附试验法测定两组患者血清VEGF及其sFlt-1水平。结果子痫前期组孕晚期及临产前VEGF水平显著低于正常对照组(t值分别为-160.762、22.690,均P<0.001),而sFlt-1水平显著高于正常对照组(t值分别为18.647、47.782,均P<0.001)。轻度子痫前期组孕晚期及临产前VEGF水平显著高于重度子痫前期组(t值分别为4.869、4.626,均P<0.05),而sFlt-1水平显著低于重度子痫前期组(t值分别为-3.59、-3.681,均P<0.05)。两组产后1周VEGF和sflt-1水平比较无显著性差异(t值分别为3.061、1.760,均P>0.05)。结论子痫前期患者血清中sFlt-1水平于孕晚期及临产前较正常孕妇明显增高,而VEGF水平降低,提示VEGF、sFlt-1可作为诊断子痫前期的指标之一。

Objective To investigate the serum levels and significance of vascular endothelial growth factor （ VEGF ） and its receptor soluble fms-like tyrosine kinase receptor 1 （ sFlt-1 ） in pregnant women with preeclampsia .Methods Totally 60 pregnant women with preeclampsia were selected in study group during the period of August 2011 to March 2012, including 30 cases with mild preeclampsia and 30 with severe preeclampsia .Another 30 normal women in late pregnancy were selected in control group .The levels of VEGF and sFlt-1 in two groups were detected by enzyme-linked immunosorbent assay （ELISA）.Results The serum levels of VEGF in preeclampsia group at late pregnancy and postpartum were significantly lower than those in control group （t value was -160.762 and 22.690, respectively, both P〈0.001）, but the serum levels of sFlt-1 were significantly higher than those in control group （ t value was 18.647 and 47.782, respectively, both P〈0.001）.The serum levels of VEGF of mild preeclampsia group at late pregnancy and postpartum were significantly higher than those in severe preeclampsia group （t value was 4.869 and 4.626, respectively, both P〈0.05）.However, the serum levels of sFlt-1 of mild preeclampsia group were significantly lower than those of severe preeclampsia group （ t value was -3.59 and -3.681, respectively, both P〈0.05）.There was no significant difference in the levels of VEGF and sFlt-1 one week after delivery between two groups （t value was 3.061 and 1.760, respectively, both P〉0.05）.Conclusion The level of sFlt-1 in serum of preeclampsia patients at late pregnant stage is significantly higher than normal pregnant women , whereas the VEGF level goes down .VEGF as well as sFlt-1 can be used as the indicators for diagnosing preeclampsia .