不同方药对大鼠急性酒精性胃黏膜损伤炎性因子的影响

Impacts of Different Formulas on Inflammatory Factor in the Rats with Acute Alcoholic Gastric Mucosal Damage

目的研究中医不同治法及复方对大鼠急性酒精性胃黏膜损伤的预防和治疗效果。方法建立酒精所致大鼠急性胃黏膜损伤的模型,用ELASE法测定胃黏膜组织匀浆后的白细胞介素-2(IL-2)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)以及肿瘤坏死因子(TNF)含量的变化。结果柴胡疏肝散组、保和丸组、黄芪建中汤组分别能升高15、30及60 min大鼠胃液中IL-2含量。保和丸组30 min时大鼠胃液中IL-6含量最高。黄芪建中汤组和丹参饮合失笑散组均能显著降低酒精作用15 min后大鼠胃液IL-10含量。酒精损伤30及60 min时,各个方药组大鼠胃液中IL-10含量均显著降低,其中作用最强的是丹参饮合失笑散组。酒精作用15、30及60 min时各个方药均能使大鼠胃液TNF含量显著降低,其中15 min时黄芪建中汤组作用最强,30 min时丹参饮合失笑散组作用最强,60 min时黄芪建中汤组作用最强。结论不同方证中药在抗炎、降低炎性介质等方面均可起到有效作用,其结果的不同可为临床治疗急性酒精性胃黏膜损伤性疾病提供科学的选药组方规律以及指导意义。

Objective To study the preventive and therapeutic effects on acute alcoholic gastric mu-cosal damage treated with different methods and compound formulas of TCM in the rats. Methods The rat model of alcohol - induced acute gastric mucosal damage was prepared. ELASE was used to determine the levels of IL - 2,IL - 6,IL - 10 and TNF in the gastric mucosal tissue homogenate. Results IL - 2 level in gastric juice was increased in 15min,30min and 60min in Chaihu Shugan San group,Baohe Wan group and Huangqi Jianzhong Tang group separately. IL - 6 level was the highest in 30min in Baohe Wan group. IL - 10 level was decreased significantly in 15min in Huangqi Jianzong Tang group and the mixed group of Danshen Yin and Shixiao San. In 30min and 60min after alcoholic damage,IL - 10 level was decreased significantly in each formula group,in which,the effect was the strongest in the mixed group of Danshen Yin and Shixiao San. In 15min,30min and 60min after alcoholic effect,TNF level was decreased significantly in each group,in which,the effect was the strongest in 15min in Huangqi Jianzhong Tang group,it was the strongest in 30min in the mixed group of Danshen Yin and Shixiao San and was the strongest in 60min in the Huangqi Jianzhong Tang group. Conclusion All of the formulas are effective in anti - inflammation and reducing the levels of inflammatory factors. The different effects provide the rules and guides for the scientific composition of formu-la in the clinical treatment of acute alcoholic gastric mucosal damage.