非霍奇金淋巴瘤中p73基因甲基化与mRNA表达的相关性研究

Studies on Relativity of p73 Gene Methylation and Expression of p73 in Non-Hodgkin Lymphoma

目的:探讨NHL中p73基因启动子甲基化与其mRNA表达的相关性。方法:用甲基化特异性PCR(MSP)方法对24例非霍奇金淋巴瘤(NHL)p73基因启动子的甲基化状况进行检测,用RTPCR检测其mRNA表达。结果:24例NHL中21例发生甲基化,甲基化阳性率87.5%;对照组均未出现甲基化。24例NHL中20例p73mRNA阴性表达,占83.33%,对照组p73mRNA表达阳性。结论:在NHL中p73mRNA表达下调,且p73基因表达与p73基因启动子的甲基化呈显著负相关;p73基因启动子的甲基化可能是造成p73基因表达下调进而导致NHL发病的原因之一。

Objective： To investigate the relativity of p73 gene methylation and expression of p73 in Non-Hodgkin lymphoma. Methods.. the methylation status of p73 gene promoter and the expression of the p73 gene mRNA was examined in 24 NHLs by methylation-specific PCR（MSP） and reverse transcription polymerase chain reaction （RT-PCR） respectively. Results：methylation-specific PCR（MSP） reveals that the promoter of p73 was methylated in 21 （87.5 %） of 24 NHLs and not methylated in all of 12 reactive hyperplasia and 22 health adult peripheral blood, p73 mRNA was not expressed in 20 （83.33%） of 24 NHLs and expressed in all of 12 reactive hyperplasia and 22 health adult peripheral blood. Conclusion.. Downregulation of p73 mRNA may be concerned with the pathogenesis of NHLs and that methylation is the predominant mechenism of inactivation of p73 gene in NHL.