序贯联合应用HIV-1 gp160腺病毒载体疫苗的体液免疫效果研究

Study on humoral immune responses induced by immunization with Ad5-HIVgp160 alone or combined with other HIV vaccine

目的探讨序贯联合应用Ad5-HIVgp160疫苗与其他艾滋病疫苗所诱导的特异性体液免疫反应效果。方法用Ad5-HIVgp160疫苗、其他腺病毒载体和腺病毒相关载体疫苗以不同免疫程序分别免疫豚鼠,通过ELISA和中和实验方法检测其诱导的免疫反应,比较疫苗联合免疫组豚鼠与疫苗单独免疫组豚鼠诱导的体液免疫反应的强度。结果不同免疫程序的豚鼠均能检测到针对HIV gp120的HIV结合抗体和针对HIV敏感株sf162的中和活性;联合疫苗组诱导的gp120特异性抗体滴度和病毒中和活性高于单独免疫组,但无显著性差异。结论 Ad5-HIVgp160疫苗单独或与其他疫苗联合使用均能诱导高水平的体液免疫反应,能激发针对HIV敏感株产生中和活性的抗体。

Objective To study humoral immune response of Ad5-HIVgpl60 combined immunization strategy. Methods Guinea pigs were injected with Ad5-HIVgpl60 alone or combined with other HIV vaccine. Humoral immune response was detected by ELISA or neutralization assay. Results Compared with immunization of Ad5-HIVgpl60 alone, anti-gpl20 antibody titer and neutralizing antibody activity were slightly increased in combination group. Conclusion Ad5-HIVgpl60 induces robust humoral immune response alone or combined with other HIV vaccine.