IL-6对大鼠骨髓间充质干细胞VCAM-1表达的影响

The effect of IL-6 on expression of VCAM-1 in rat marrow mesenchymal stem cells

目的探讨白介素6(IL-6)对大鼠骨髓间充质干细胞(rMSCs)血管细胞黏附分子(VCAM-1)表达的影响。方法将培养的rMSCs分为两组,各组均设6个样本:一组用终浓度为10ng/ml的IL-6刺激12h,另一组不加细胞因子刺激;然后采用免疫细胞化学方法检测两组细胞VCAM-1蛋白的表达,采用免疫荧光直标法检测两组细胞表面VCAM-1抗原的表达率,RT-PCR法检测两组细胞VCAM-1mRNA的表达。结果免疫细胞化学染色显示,未刺激组细胞染色浅,呈淡棕黄色;IL-6刺激组细胞呈棕褐色。流式细胞仪检测显示,未刺激组VCAM-1的表达率为14.1%;IL-6刺激组VCAM-1的表达率为80.9%。RT-PCR显示,未刺激组VCAM-1mRNA呈微弱表达;刺激组VCAM-1mRNA的表达显著增强(P<0.01)。结论 IL-6能诱导rMSCs VCAM-1的表达显著增高,提示VCAM-1可能在MSCs替代治疗初始阶段发挥重要作用。

Objective To study the effect of IL-6 on the expression of VCAM-1 in rat marrow mesenchymal stem cells （rMSCs）.Methods rMSCs were divided into two groups： the cells of one group were treated with IL- 6 （10ng/ml） for 12h and the other were regarded as control. VCAM-1 protein was identified by using immunocyto- chemical methods,the antigen of VCAM-1 were detected by flow cytometry and their mRNA were measured with RT-PCR.Results Immunocytochemistry and RT-PCR showed that low levels of VCAM-1 were expressed in the rMSCs,and flow cytometry showed that the rate of VCAM-1 expression was 14.1% in control group, which was 92.8% after pretreatment with IL-6.Conclusion IL-6 induced a concentration-dependent increase in VCAM-1 expression on MSCs. So we can see that VCAM-1 possibly plays an important role in the process of MSCs replacement therapy.