棕榈科植物DNA的提取及SRAP引物筛选

Extraction of Genomic DNA from Palm Plants and Screening of SRAP Primers

采用CTAB法提取棕榈科植物基因组DNA,选用4个棕榈科植物DNA作模板,对100个SRAP引物组合进行筛选,以条带清晰多态性丰富为引物筛选原则,最后共筛选出14个组合作为棕榈科植物SRAP分子标记的核心引物,为后续SRAP分析提供依据。同时还对SRAP-PCR扩增程序进行优化,在不影响扩增效果的前提下,节约了大量实验时间。

The genomic DNAs were extracted from palm plants by using CTAB method , and 100 pairs of SRAP primers were screened for clear bands and rich polymorphism by using the extracted genomic DNAs from four palm plants as templates .Finally, 14 pairs of SRAP primers were screened out and were used as the core primers of palm plants SRAP molecular markers , which pro-vided the basis for the subsequent SRAP analysis in palm plants .This study also optimized the amplification program of SRAP -PCR, which could save much experimental time on the premise of not affecting the amplification effect .