期刊文献+

敲除MIG1同时过表达MAL62面包酵母的发酵性能 被引量:2

Fermentation Abilities of the Recombined Strain BPM-M and its Overexpressing MAL62 along with Deletion of MIG1
下载PDF
导出
摘要 以亲本面包酵母工业菌株BY-14a为对照,研究了敲除MIG1同时过表达MAL62面包酵母重组菌BPM-M的发酵性能.实验结果表明:重组菌株BPM-M的麦芽糖酶活提高,麦芽糖利用速率较亲本菌株提高29.1%;葡萄糖阻遏减弱38.8%;无糖面团发酵力达到163.4mL/(h·g),较亲本菌株提高了50.6%. The fermentation abilities of the recombined strain BPM-M and its overexpressing MAL62 along with deletion of MIG1 were investigated. Our results showed that the recombined strain BPM-M has the following advantages over the parental strain BY-14a. Maltose utilization was 29.1% higher, glucose repression of BPM-M was reduced by 38.8%, and the leavening ability of the lean dough was enhanced to 163.4 mL/(h·g), which was 50.6% higher than that of BY-14a.
出处 《天津科技大学学报》 CAS 2014年第3期1-5,共5页 Journal of Tianjin University of Science & Technology
基金 国家自然科学基金资助项目(31000043 31171730)
关键词 面包酵母 MAL62 MIG1发酵性能 无糖面团 baker's yeast MAL62 MIG1 fermentation ability lean dough
  • 相关文献

参考文献16

  • 1Bell P J, Higgins V J, Attfield P V. Comparison of fer- mentative capacities of industrial baking and wild-type yeasts of the species Saccharomyces cerevisiae in differ- ent sugar media[J]. Letters Applied Microbiology, 2001,32 (4) : 224-229.
  • 2李红民,黄仁泉.激光诱变选育面包酵母菌的研究[J].光子学报,2001,30(11):1381-1384. 被引量:6
  • 3Salema-Oom M, De Sousa H R, Assuncao M, et al. Derepression of a baker's yeast strain for maltose utiliza- tion is associated with severe deregulation of HXT gene expression [J]. Journal of Applied Microbiology, 2011, 110 (1) : 364-374.
  • 4Higgins V J, Braidwood M, Bell P, et al. Genetic evide- nce that high noninduced maltase and maltose permease activities, governed by MALx3-encoded transcriptional regulators, determine efficiency of gas production by baker's yeast in unsugared dough[J]. Applied and Environmental Microbiology, 1999,65 (2) : 680-685.
  • 5Hu Z, Gibson A W, Kim J H, et al. Functional domain analysis of the Saccharomyces MAL-activator[J]. Cur- rent Genetics, 1999,36 (1/2) : 1-12.
  • 6Jiang Tianxiao, Xiao Dongguang, Gao Qiang. Characteri- sation of maltose metabolism in lean dough by lagging and non-lagging baker's yeast strains [J]. Annals of Mi- crobiology, 2008,58 (4) : 655-660.
  • 7Sun X, Zhang C, Dong J, et al. Enhanced leavening prop-erties of baker's yeast overexpressing MAL62 with dele- tion of MIG1 in lean dough [J]. Journal of Industrial Mi- crobiology & Biotechnology, 2012,39 (10) : 1533-1539.
  • 8Nehlin J O, Carlberg M, Ronne H. Control of yeast GAL genes by MIG1 repressor: A transcriptional cascade in the glucose response[J]. EMBO Journal, 1991, 10 (11) : 3373-3377.
  • 9Cao H L, Yue M, Li S G, et al. The impact of MIG1 and/or MIG2 disruption on aerobic metabolism of succi- nate dehydrogenase negative Saccharomyces cerevisiae [J]. Applied Microbiology and Biotechnology,2011, 89 (3) : 733-738.
  • 10Horák J. Regulations of sugar transporters : Insights from yeast[J]. Current Genetics, 2013,59 (1/2) : 1-31.

二级参考文献8

共引文献5

同被引文献9

引证文献2

二级引证文献6

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部