灰葡萄孢菌Rac1基因的生物信息学分析

Bioinformatics Analysis of Botrytis cinerea Rac 1 Gene

利用多种不同序列分析软件,基于生物基因组学数据库,对灰葡萄孢菌Rac1基因进行生物信息学分析,以预测BCRac1基因编码产物的理化性质、结构与功能,同时构建Rac1基因同源系统进化树。结果表明:BCRac1基因编码产物由150个氨基酸构成,为亲水性蛋白,没有信号肽,二级结构以α-螺旋与无规则卷曲为主,推测其主要在细胞核中发挥生物学作用。结构功能域分析表明,BCRac1基因编码产物可能主要具有生长因子的功能,对灰葡萄孢菌的激素调节有影响。构建的Rac1基因编码产物系统进化树显示,BCRac1基因与核盘菌、稻瘟病菌、炭疽菌等物种Rac1基因遗传距离较近,具有高度同源性。

This study aimed to analyze the bioinformatics of BCRac1 gene in Botrytis cinerea with genomics database. The physicochemical characteristics, structures and functions of the coding product of Botrytis cinerea BCRac1 gene were predicted and analyzed. Meanwhile,the phylogenetic tree of Rac1 and related genes was constructed. The results showed that BCRac1 gene encoded a hydrophilic protein of 150 aa,which had no signal peptide. The secondary structure was primarily composed of alpha helix and random coil, and the biological functions of BCRac1 speculatively performed in the nucleus. Sequence analysis indicated that the coding product of BCRad might mainly act as growth factor, and play an important role in the hormonal regulation of Botrytis cinerea. The phylogenetic tree demonstrated that the BCRac1 had a close genetic distance and high homology with Sclerotinia sderotiorurn ,Magnaporthe grisea and Colletotrichurn gloeosporioides.