小鼠PE右心室导管的制作及应用研究

Design and application research of PE right ventricular catheter for mice

目的建立一种制作简单、成本低廉、性能稳定的小鼠右心室压力检测方法。方法选取一段长度约为15cm的PE-50导管（外径：0．9mm，内径：0．5mm），将一端制成适宜弧度，另一端插入7号注射器针头连接压力换能器。选取80只SPF级雄性C57BL／6小鼠，用自制PE右心室导管行右侧颈外静脉插管至右心室，记录成功例数和每次操作时间。另取40只SPF级雄性C57BL／6小鼠随机分为对照组和慢性低压低氧组，每组20只。慢性低压低氧组小鼠在模拟海拔5000m低压舱内不间断喂养4周，对照组在舱外同时喂养。模型复制成功后，用自制PE右心室导管行右心室插管，记录右心室收缩压；分离左、右心室并称质量，计算Hermann-Willson指数并与右心室收缩压进行相关性分析。结果采用此自制PE右心室导管，右心室插管成功率为90％（72／80），从分离血管至检测出右心室波形所需时间每只3～5rain。慢性低压低氧组右心室收缩压（39．52±4．34）mmHg和Hermann-Willson指数0．356±0．039均显著高于对照组（21．24±2．70）mmHg、0．256土0．020，二者呈显著正相关（P〈0．01）。结论采用本法检测右心室压力，制作简单，使用方便快捷，成功率高，性能稳定且成本低廉，可以推广应用。

Objective To establish a simple,low cost and stable method to detect right ventricular pressure in mice. Methods A PE-50 duct length 15 cm（outside diameter：0.9 ram,inner diameter,.0.5 mm） was bent on one terminal and the other terminal was inserted into a 7 =syringe needle to connect to a pressure transducer. This duct was intubated into right ventricle via right external jugular vein to detect right ventricular pressure in 80 SPF grade male C57BL/6 mice. Successful cases and operation time were recorded. Besides,40 SPF grade male C57BL/6 mice were randomized into the control group（n= 20） and chronic hyperbaric hypoxia group（n= 20）. Mice in chronic hyperbaric group were raised in a hyperbaric chamber of simulated 5 000 m high altitude {or 4 weeks. The control group was raised outside the chamber simultaneously. Right ventricular systolic pressure was detected with the PE duct. Left and right ventricles were detached and weighed,and Hermann-Willson index was calculated. Results With this PE duct, right ventricular intubation success rate was 90 % （72/80）, the operation cost approximately 3 to 5 min each mouse from the separation of blood vessels to detect the time needed for the right ventricle waveform, right ventricular systolic pressure[（39.52± 4.34 ）mm Hg] and Hermann-Willson index（0. 356 ± 0. 039）of chronic hyperbaric hypoxia group were significantly higher than that of control group [-（21.24±2.7）mm Hg and （0. 256±0. 020）] ,which has significant positive correlation （P〈0.01）. Conclusion It is simple, fast,stable,costing low and of high success ratio to detect right ventricular pressure with this method.