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基质金属蛋白酶-14在骨关节炎滑膜、滑液中的表达及研究 被引量：3

Expression and significance of matrix metalloproteinase 14 in synovial tissue and syrovial fluid of osteoarthritis
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摘要 目的探讨基质金属蛋白酶-14(MMP-14)在骨关节炎(OA)患者滑膜、滑液中的表达及意义。方法采用半定量RT-PCR检测32例OA患者(实验组)及26例非OA患者(对照组)关节滑膜组织中MMP-14mRNA的表达水平;采用ELISA法检测关节滑液中MMP-14蛋白的表达水平。结果经RT-PCR检测MMP-14mRNA在实验组与对照组患者滑膜组织中的表达差异有统计学意义(P<0.05),ELISA检测两组滑液中MMP-14蛋白表达差异有统计学意义(P<0.05)。结论 MMP-14在OA滑膜中呈高表达,表明在OA的发生及发展过程中可能起促进作用;在滑液中低表达,表明OA发生时MMP-14未大量释放至滑液,间接表明MMP-14的主要功能于滑膜细胞中表达并促进骨关节炎的发展。 Objective To evaluate the expression and clinical significance of matrix metalloproteinase 14（MMP-14） in osteoarthritis（OA） patients in synovium and synovial fluid of knee joint. Methods Semi-quantitative RT-PCR were used to detect the expression of MMP-14 mRNA in 32 case of OA （experimental group）and 26 non-OA patients（control group）synovium; protein level expression of MMP-14 in synovial fluid were analyzed by ELISA. Results The expression of MMP-14 mRNA in experimental group synovium of knee joint was significantly increased compared with control group（P〈0.05） ;but the protein level of MMP-14 was low in experimental group synovial fluid compared with control group（P〈0.05）. Conclusion The expression of MMP-14 is increased in OA patients synovium and maybe promote the progress of OA directly; the protein level of MMP-14 is decreased in OA synovial fluid,indicated the function of MMP-14 in OA synoviocyte indirectly.
作者 郑伟 史晨辉 王维山 霍红军 李保驰
机构地区 石河子大学医学院第一附属医院骨一科
出处 《重庆医学》 CAS CSCD 北大核心 2014年第19期2417-2419,共3页 Chongqing medicine
基金 国家自然科学基金资助项目(30960387)
关键词 骨关节炎 基质金属蛋白酶14 逆转录聚合酶链反应 酶联免疫吸附测定 osteoarthritis matrix metalloproteinase 14 RT-PCR ELISA
分类号 R684.3 [医药卫生—骨科学]
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参考文献14

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二级参考文献13

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重庆医学
2014年 第19期

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