改良抗体结合试验检测人用狂犬病疫苗效价方法的初步建立

Initial Establishment Method to Detect the Potency of Rabies Vaccine for Human Use by Modified Antibody Binding Test

目的建立改良抗体结合试验(Modified Antibody Binding Test,MABT)的检测流程,并试用于人用狂犬病疫苗(Rabies Vaccine for Human Use,RabV)的效价检测。方法由省级疾病预防控制中心(Center for Disease Control and Prevention,CDC)提供RabV样品,采用制订的MABT流程测定RabV样品效价。结果 MABT检测可以显示RabV样品效价的相对高低,A和B两个RabV样品经MABT测定效价均为4.4国际单位(International Unit,IU)/毫升(ml)(>2.5IU/ml)。结论制订的MABT检测流程工作稳定,MABT可以用于RabV效价的辅助检测。

Objective To establish the protocol of modified antibody binding test （MABT） and try to detect the potencies of Rabies Vaccine for Human Use （RabV） by MABT. Methods RabV samples were offerd by provincial center for disease control and prevention. The potencies of RabV samples were detected by the formulated protocol of MABT. Results The relatively comparison of the potencies of RabV samples was reflected in MABT. The potencies of A and B RabV samples were all detected as 4.4 International Unit （IU）/ml （ 〉 2. 5IU/ml） by MABT. Conclusion The formulated protocol of MABT worked stable. MABT could be assisted to detect the potencies of RabV samples.