一种预染色凝胶电泳法的研究

Research on pre-staining gel electrophoresis

凝胶电泳是常用的生化分离鉴定技术。生物分子大多在可见光区完全透明,因此在凝胶电泳之后,需要对凝胶进行染色处理才能观察到结果,而且常规的染色方法一般步骤比较繁琐耗时。本文开发了一种新型的预染色凝胶电泳方法(Pre-Staining Gel Electrophoresis,PSGE),可以直观地显示电泳结果,并简化凝胶电泳操作。利用PSGE成功分析了蛋白质与两性高分子聚合物的共价偶联和物理吸附的效率,展示了PSGE的巨大应用开发潜力。

Background： Gel electrophoresis is a powerful biochemical separation technique. Most biological molecules are completely transparent in the visible region of light, so it is necessary to use staining to show the results after gel electrophoresis, and the general steps of conventional staining methods are time-consuming. Purpose： We try to develop a novel approach to simplify the gel electrophoresis： Pre-Staining Gel Electrophoresis （PSGE）, which can make the gel electrophoresis results monitored in real time. Methods： Pre-stain the protein samples with Coomassie Brilliant Blue （CBB） for 30 rain before loading the sample into the gel well. Results and Conclusion： PSGE can be successfully used to analyze the binding efficiency of Bovine Serum Albumin （BSA） and amphiphilic polymer via chemical coupling and physical absorption, and the double PSGE also shows a great potential in bio-analytical chemistry.