HSV-TK/ACV系统促肾癌细胞株GRC-1细胞凋亡的实验研究

Induction of apoptosis by herpes simplex virus-thymidine kinase and aciclovir therapy in renal carcinoma cell lines GRC -1

目的 观察HSV TK/ACV系统对肾癌GRC 1细胞的杀伤作用及旁观者效应。方法 将含不同浓度的肾癌GRC 1/TK细胞与肾癌GRC 1细胞混合培养 ,分为 4组 ,分别含GRC 1/TK细胞 0 %、2 5 %、5 0 %及 10 0 % ,分别用无环鸟苷 6 0 μg/ml进行处理 ,观察细胞形态改变、测定活细胞数并行流式细胞术、电子显微镜检查。结果 实验组与对照组GRC 1细胞均无明显变化 ;含 2 5 %以上GRC 1/TK细胞可观察到明显的杀伤作用 ,并有凋亡峰及凋亡小体形成。结论 HSV TK/ACV系统对肾癌GRC 1细胞具有明显的杀伤作用及旁观者效应 。

Objective To study bystander effect and cell death by HSV TK and ACV therapy on renal carcinoma cell lines GRC 1. Methods Different mix culture cells, which contained GRC 1/TK cells 0%、25%、50% and 100%,were treated with ACV 60 μg/ml, the morphology and quantity of tumor cells being studied and checked with FCM and electron microscope. Results After exposure to ACV, GRC 1/TK cells, even only 25%, underwent cell death and bystander effect, but there was no obvious change in the morphology and quantity of GRC 1 cells exposed to ACV. Conclusions There were bystander effect and cell death on HSV TK and ACV therapy in renal carcinoma cell lines GRC 1. The mechanism of bystander effect may have deat with apoptosis.