摘要
目的 :探讨血清饥饿以及EGF对人肝癌细胞EGFR表达的影响。方法 :用免疫组化方法研究血清饥饿、5ng/mlEGF和 2 5ng/mlEGF对人肝癌细胞EGFR表达的影响。 结果 :撤血清培养组、5ng/mlEGF和 2 5ng/mlEGF刺激组人肝癌细胞EGFR的表达阳性细胞分别为 ( 5 66± 0 .86) %、( 10 67± 1.2 6) %和 ( 16 83± 1.66) % ,与正常对照组 ( 1 81± 0 .75) %相比较差异显著 (P <0 0 1) ,EGF刺激组比血清饥饿组明显增高 (P <0 0 1)、2 5ng/mlEGF组比 5ng/mlEGF组增高 (P <0 0 1)。 结论 :血清饥饿和EGF能明显增强人肝癌EGFR的表达 ,且EGF上调EGFR表达的作用具有剂量依赖效应。
Objective:To investigate the effect of serum starvation on expression of EGFR and the effect on expression of EGFR by EGF in human hepatocellular carcinoma cells.Methods:Human hepatocellular carcinoma cells were treated with serum free culture medium and the concentration of the 5ng/ml EGF,25ng/ml EGF in culture medium respectively for six hours,and then the expression of EGFR in BEL7402 human hepatocellular carcinoma cells was studyed by immunohistochemistry technique.Results:Our results indicate that the positive cells of EGFR expression in human hepatocellular carcinoma cells were(5.66±0.86)%、(10 67±1 26)% and (16.83±1.66)% respectively.Compared with normal control group(1.81±0.75)%,the EGFR expression of theirs was significantly higher than that of control group while the positive cells of the group treated with EGF were more higher than that of group cultured in serun free culture medium,and also the expression of EGFR of group treated with the concentration of the 25ng/ml EGF was higher than that of group treated with the concentration of the 5ng/ml EGF.Conclusion:Serum starvation and EGF can enhance the expression of EGFR in human hepatocellular carcinoma obviously.The up regulation of EGFR expression by EGF in HCC cells was showed dose depended.
出处
《山东医科大学学报》
2001年第1期21-23,共3页
Acta Academiae Medicinae Shandong
关键词
血清饥饿
表皮生长因子
尿抑胃素
受体
体外研究
肝细胞癌
Serum starvation
Carcinoma,hepatocellular
Epidermal growth factor urogastrone
Receptors,epidermal growth factor urogastrone
In vitro