微波辅助提取超微毛木耳粉多糖的工艺优化

Optimization of Microwave Assisted Extraction of Polysaccharides from Superfine Ground Powder of Auricularia Polytricha

采用响应面优化法来确定微波辅助提取毛木耳超微粉多糖的最佳工艺条件。通过单因素试验探讨水料比、微波功率、微波时间这3个因素对毛木耳超微粉中多糖提取效果的影响,根据单因素试验结果,以水料比、微波功率和微波时间进行三因素三水平的响应面试验,依据回归分析得到最优工艺条件为:水料比60 mL/g,微波功率600W,微波时间2.0 min。此条件下毛木耳超微粉中多糖的提取得率为28.78%。

In this study, response surface methodology was employed to optimize the extraction of polysaccharides from superfine powder of Auricularia Polytricha. The single experiments were used to explore the effects of different ratio of water to raw material, microwave power, microwave time on the yield of polysaccharides. A box-behnken design was adopted to determine optimum parameters （ratio of water to raw material, microwave power, microwave time） that could yield a maximum polysaccharide. Based on the regression analysis, the optimum extraction conditions were as follows：ratio of water to raw material 60 mL/g, microwave power 600 W, microwave time 2.0 min. Under this optimized conditions, the experimental yield of polysaccharides of Auricularia Polytricha was 28.78%, which was consistent with the predicted model.