南极磷虾中氟含量的两种测定方法优化与比较

Comparison of Two Methods for the Determination of Fluorine Content in Antarctic Krill

采用灰化蒸馏-氟试剂比色法(ADFRC)和离子选择电极法(ISE)测定南极磷虾中的氟含量,对两种方法的检测条件进行优化,采用精密度和t检验比较两种方法检测结果的差异性。结果表明,ADFRC法的最适波长为580 nm,样品馏出液稀释25倍测定样品中氟含量;ISE法采用标准溶液添加法绘制标准曲线,取样2 g,加盐酸10 mL,测定样品中氟含量。与ADFRC法相比,ISE法操作简单快速,样品平行性好。利用ISE法测定南极磷虾各部位及其制品中的氟含量,可见,南极磷虾中氟主要蓄积在甲壳及头部,在加工过程中去掉甲壳及头部可降低南极磷虾制品中的氟含量。

Ashing distillation-fluorine reagent colorimetric method （ADFRC） and ion-selective electrode method （ISE） were used to determine the fluoride content in Antarctic krill. Results of two methods were analyzed by precision and t test. The results showed that the optimum wavelength of ADFRC method was 580 nm, and the sample distillate dilution was 25 times. The standard curve of ISE method was determined by standard solution adding method. ISE sampling was 2 g, hydrochloric acid 10 mL to determine the fluorine content in the sample. ISE method was proved simple and rapid in operation and better precision than ADFRC method. ISE method was used to determine the fluorine content in Antarctic krill and its products. Statistics analysis indicated that fluoride mainly accumulated in shell and head of Antarctic krill, and the fluoride content in Antarctic krill products could be reduced by removing shell and head during processing process.