明胶抗氧化肽的分离、纯化及其清除羟自由基活性的研究

Study of Scavenging Effecton on OH Radical,Separation and Purification of Gelatin Antioxidant Peptides

为制备具有抗氧化活性的明胶肽,本实验采用不同的酶水解明胶,并对酶解产物采用超滤技术分离,利用分光光度法检测超滤后所得多肽组分对羟基自由基(·OH)的清除能力;进一步对抗氧化活性较强的组分利用葡聚糖Sephadex C-25阳离子交换色谱、Sephadex G-50凝胶过滤色谱和C18反向高效液相色谱进行分离、纯化,并对其各组分清除·OH活性进行了研究,最后对所得洗脱组分中具有最强清除自由基活性的多肽组分的分析氨基酸组成,结果显示:组分中甘氨酸、蛋氨酸、苯丙氨酸、赖氨酸含量较高的肽具有较好的抗氧化活性,·OH清除率达到55.37%。

We used four different kinds of commercial protease to hydrolyze gelatin, and measure the antioxidant activity of hydrolysate such as their abilities to scanvage OH· radicals. Peptides derived from geltain was separated by ultra-filtration technology. The result showed that the trypsin hydrolysate had the best antioxidant activity. Furthermore, we used SP-Sephadex C-25 cation exchange chromatography and Sephadex G-50 gel filtration chromatography to separate the trypsin hydrolysate and get an elution peak part with strong activities of scanvaging OH·radicals. The result of experiment indicates that the OH·scavenging rate of the hydrolyzate was up to 55.37%. They contained the key amino acid for the antioxidation, such as Glu,Met,Phe,Lys et al.