红葡萄酒中合成色素的测定研究

Study on Determination of Synthesis Colorants in Red Wine

建立了HPLC测定红葡萄酒中色素柠檬黄、苋菜红、日落黄、胭脂红含量的方法，采用色谱柱：THERMO C18（4.6 mm×250 mm，5μm），检测波长：254 nm，流量：1.0 mL/min，进样量：10μL，流动相：0.02 mol/L乙酸铵与甲醇。梯度条件为：甲醇20%-35%，3%/min；35%-98%，9%/min；98%继续6 min。结果表明：柠檬黄、苋菜红、胭脂红、日落黄在0-50μg/mL范围内呈现良好的线性关系，Y=30264440X＋3047.2（R=0.9998）、Y=29741606.67X＋607.03（R=0.9998）、Y=28790266.67-6523.42（R=0.9998）、Y=25122776.67X＋1953.58（R=0.9998）。色素在24 h内稳定。本方法简单，快速、准确、重现性好，可以用作葡萄酒中色素的含量测定。

To establish high performance liquid chromatography （HPLC） method for determination content of colorants in red wine, include Lemon yellow, Amaranth, Sunset yellow, Carmine. Thermo C18 column was used and the mobile phase was 0.02 mol/L ammonium acetate and methanol, flow rate was 1.0 mL/min, wavelength was UV at 254 nm and the sample volume was 10μL. Gradient conditions：methanol：20%-35%,3%/minutes;35%-98%,9%/minutes;98%remain 6 minutes. The results show that the Lemon yellow, Amaranth, Sunset yellow, Carmine concentration had good liner relationship from 0-50μg/mL, Y=30 264 440X＋3 047.2 （R=0.999 8）,Y=29 741 606.67X＋607.03（R=0.999 8）,Y=28 790 266.67-6 523.42（R=0.999 8）,Y=25 122 776.67X＋1 953.58（R=0.999 8）. The colorants were stability in 24 hours. The method was simple, fast, accurate, and precise, could be use to determine content of colorants in red wine.