半仿生提取柿叶黄酮及其抗氧化和抗菌作用

Semi-bionic Extraction of Total Flavonoids from Persimmon Leaves and Its Antioxidant and Antibacterial Effects

对磨盘柿叶黄酮的半仿生法提取工艺及其体外抗氧化和抗菌作用进行研究。通过正交试验确定半仿生法提取磨盘柿叶总黄酮的最佳工艺条件:固液比1 g:20 mL,提取温度80℃,提取时间1 h,分别以pH≈2.0盐酸溶液和pH≈7.5、pH≈8.5的氨-氯化氨缓冲溶液作为提取液各提取1次。实验表明柿叶总黄酮对油脂过氧化、DPPH自由基、羟基自由基、超氧自由基均有一定的抑制作用。采用滤纸片实验抑菌作用,结果表明磨盘柿叶总黄酮溶液对白葡萄菌和大肠杆菌均有中度抑菌作用。

The semi-bionic extraction of total flavonoids from mopan persimmon leaves and its antioxidant and antibacterial effect in vitro were studied. The optimum process based on the orthogonal test was as the following：using pH≈2.0 HCl solution, pH≈7.5 and pH≈8.5 NH3·H2O-NH4Cl buffer solution as extracting solvent, extracted one time of each at the rate of solid to solvent was 1 g：20 mL, and 80℃for 1 h. The inhibitory actions on DPPH free radical, hydroxyl radical and superoxide radical of total flavonoids from mopan persimmon leaves were confirmed in this experiment. Meanwhile, moderate antibacterial effect of against White staphylococcus and Escherichia coli were abserved through filter paper experiments.