正交试验法优选红茶中儿茶素及咖啡碱的提取工艺

Extracting Technique for Catechin and Caffeine from Selected Red Tea by Orthogonal Test

优选了红茶中咖啡碱、儿茶素（C）、表儿茶素（EC）、表没食子儿茶素没食子酸酯（EGCG）、表儿茶素没食子酸酯（ECG）的最佳提取工艺。通过正交试验法考察了提取时间、甲醇体积分数、料液比对红茶中儿茶素及咖啡碱质量分数的影响。试验发现红茶的最佳提取工艺条件为：提取时间20 min，体积分数为80％的甲醇溶液，料液比为m（红茶）∶V（甲醇）＝1∶50（g/mL）。

The best extraction technique is used for caffeine,catechin （C ）,[（-）-epicatechin （EC ）,（-）-epigallocatechin gallate （EGCG）,（-）-epicatechin gallate（ECG）from selected red tea.The extraction time,methanol concentration and the affect of material-to-liquid ratio on the mass fraction of catechin and caffeine were investigated by orthogonal experiments.Results showed that the optimal ex-traction conditions were extraction time 20 min,methanol concentration 80%and material-to-liquid ratio 1∶50 （g/mL）.