MicroRNA let-7e与食管鳞癌临床分期研究

The clinical research of microRNA let-7e expression level in human esophageal sguamous cell cancer in different clinical stages

目的:检测食管鳞癌患者不同临床分期癌组织及癌旁组织中MicroRNA let-7e表达水平的差异。方法:应用实时荧光定量RT-PCR Taqman MGB探针法,检测60例食管癌癌组织及癌旁组织中MicroRNA let-7e表达水平。以U6 snRNA为内参,进行荧光定量PCR,统计分析表达水平的差异。结果:MicroRNA let-7e的表达水平在癌组织比癌旁组织高,病理分期I期与III期相比,差异有统计学意义(P<0.05)。结论:MicroRNA let-7e在食管鳞癌中表达水平明显高于癌旁组织,可能用于食管鳞癌临床分期诊断的重要标记。

Purpose： To investigate the expression levels of MicroRNA let-7e in esophageal squamous cell cancer patients and to compare cancer tissues with adjacent normal lung tissues in different clinical stages. Method： The MicroR-NA let-7e expression levels in cancer tissues and adjacent normal lung tissues were detected by quantitative real-time re-verse transcription-PCR from 60 esophageal squamous cell cancer patients who were divided into three groups, with U6 RNA as an actin. Results： The expression levels of MicroRNA let-7e in cancer tissues were significantly higher than in adjacent normal esophageal tissues, and had statistical significance in different stages of the tumors： characteristics, lymph metastasis, differentiation, and the stage of the tumors. Conclusions： The study suggested that microRNA let-7e was sig-nificantly up-expressed in esophageal squmaos cell cancer and should be a useful biomarker for the diagnosis of clinical stages of esophageal cancer patients.