大花蕙兰丛生芽快繁体系的研究

Study on plexiform buds rapid propagation system of Cymbidium hybridum

为建立大花蕙兰丛生芽快繁体系,以大花蕙兰无菌苗假鳞茎为材料,用MS为基本培养基,进行6-BA、NAA、蔗糖和琼脂等多个正交试验进行优化筛选。结果表明:在MS+6-BA 2.5 mg/L+NAA 0.5 mg/L+蔗糖18 g/L+琼脂10 g/L+活性炭1.5 g/L的培养基上诱导出来的丛生芽效果最好,诱导率高达80.52%;在MS+6-BA 0.5 mg/L+NAA 0.4 mg/L+蔗糖25 g/L+琼脂10 g/L+活性炭1.5 g/L培养基上丛生芽增殖效果最好,增殖倍数为5.01;在MS+NAA 1.0 mg/L+蔗糖10 g/L+琼脂10 g/L+活性炭2 g/L+香蕉泥100 g/L的培养基上,培养温度为28℃时,试管苗的生根最佳。

In order to establish the rapid propagation system ofCymbidium hybridum, orthogonal experiment including the factors of 6-BA, NAA, sucrose and AGAR were carried out by taking the vaccine pseudobulb as material and using MS as the basic culture medium. The results show that the induction effect of MS ＋ 6 - BA 2.5 mg/L ＋ 0.5 mg/L NAA ＋ sucrose 18 g/L ＋ AGAR 10 g/L ＋ activated carbon 1.5 g/L is the best, and the induction rate is as high as 80.52%. proliferation effect of multiple shoot clumps on the culture medium of MS ＋ 6 - BA 0.5 mg/L ＋ NAA 0.4 mg/L ＋ sugar 25 g/L ＋ AGAR 10 g/L ＋ active carbon 1.5 g/L is the best with proliferation ratio of 5.01. on the medium MS ＋ NAA1.0 mg/L ＋ sucrose 10 g/L ＋ AGAR 10 g/L ＋ activated carbon 2 g/L ＋ banana puree 100 g/L, the tube seedlings got the best root system when the cultivating the temperature is 28℃.