食源性普罗威登斯菌的分离鉴定和耐药性研究

Isolation, Determination and Antibiotic Resistant of Food-born Providencia spp.

本文研究了食源性普罗威登斯菌在肉类食品中的的分布,以及其耐药表型与I型整合子的携带状况。本文采集了市售猪肉、鸡肉和牛肉等肉类食品,对普罗威登斯菌进行分离与鉴定;采用纸片扩散法对已分离鉴定的普罗威登斯菌进行药敏实验;利用聚合酶链式反应技术筛选携带I型整合子的菌株。结果表明,85份样品中,有38份样品检出普罗威登斯菌,检出率达44.70%。38株普罗威登斯菌中,有44.74%的分离株是多重耐药菌株,最多耐6种抗生素。有两株普罗威登斯菌携带I型整合酶,一株拉氏普罗威登斯菌携带耐药基因盒。这是在国内肉类食品分离的普罗威登斯菌中首次发现I型整合子阳性菌株,表明食品中这种携带有多重耐药的菌株有可能通过食物链向人类传播,是对人类健康造成威胁的潜在危险因素。

The objective of this study was to identify the Providencia spp. that isolated from three kinds of different meat and their antibiotic resistance performance was also investigated. API20E system was used to determine and identify the isolates from pork, chicken and beef. And Kirby-Bauer was applied to detect the antibiotic resistance ofProvidencia spp. Subsequently, Providencia spp. was screened by PCR for class I integron. The results showed that 38 samples were Providencia spp. positive in 85 samples, and the relevance ratio was 44.70%. Moreover, 44.74% of positive Providencia spp. was multiple resistant and some of them resisted up to six kinds of antibiotics. Two isolates showed class I integrase positive, and one isolate of Providencia rustigianii was found carrying gene cassette. These isolates with multiple resistances in the food may be transferred to human by food chains, and then become a potential threat for human health.