采用ISSR标记分析草珊瑚的DNA指纹图谱与其品质相关性

Analysis on DNA fingerprints of Sarcandra glabra and correlation with its quality using ISSR molecular markers

目的分析不同质量级别的草珊瑚样品的DNA指纹图谱与其品质相关性,为进一步利用这些资源进行分子辅助育种奠定一定基础。方法采用ISSR-PCR方法对UBC801-UBC900共100个ISSR引物进行筛选,筛选出多态性好的ISSR引物23个。利用这23个ISSR引物扩增18份草珊瑚样品,以18份草珊瑚样品的ISSR扩增谱带为基础,利用Excel文件建立供试材料扩增条带指纹数据库。结果共获得198条谱带,平均每个引物扩增出8条谱带,其中多态性谱带184条,多态性条带比率为92.9%。从23个多态性好的ISSR引物中遴选出UBC811、UBC825 2个引物中7个位点为一个组合,UBC827、UBC834、UBC842共3个引物中7个位点为另一个组合,分别建立了18份受试草珊瑚样品的基因组的DNA指纹图谱,并从184条多态性谱带中筛选了出u844-6条带与样品质量有一定相关性。结论 ISSR分子标记可以有效辨别18份受试草珊瑚样品的DNA指纹图谱,并筛选获得一条与其品质有一定相关性的特征条带。

Objective To analyze the correlation of DNA fingerprints of Sarcandra glabra with different quality levels and its quality.Methods Using ISSR-PCR,100 ISSR primers（UBC801—UBC900） were screened,and 23 of them were polymorphic.The 23 ISSR primers were used to amplify 18 S.glabra samples from different habitats.Based on the 18 ISSR amplified bands,the data base of amplified bands fingerprint was established using Excel.Results one hundred and ninty-eight bands were obtained.Each primer amplified eight bands on average.The number of polymorphic DNA bands was 184,and the polymorphic proportion of DNA bands was 92.9%.Seven sites in two primers（UBC811 and UBC825） screened from 23 polymorphic ISSR primers were in one group,and seven sites in three primers（UBC827,UBC834,and UBC842） were in another group.The DNA fingerprints of 18 S.glabra samples were established,and U844-6 bands were screened from 184 polymorphic bands to correlate with sample quality.Conclusion ISSR molecular markers could identify the DNA fingerprints of 18 S.glabra samples,and screen one band that is related to the quality.