红花檵木叶片再生不定芽的研究

Adventitious Bud Regeneration from Leaves of Loropetalum chinense var. rubrum Cultivars

为了建立红花檵木叶片高效的离体再生体系,解决其基因工程育种的问题,以红花檵木叶片为外植体,探讨了品种、光照条件、激素等因素对叶片离体再生的影响。结果表明:不同品种叶片再生不定芽能力不同,‘玫红细叶青’再生能力最强,其次是‘长叶玫红’,‘花叶2号’叶片再生最难。白光和红光有利于叶片再生,70天后都有不定芽出现。其中白光下的诱导率达50%,红光下诱导率达25%。叶片近轴面接触培养基,再生效率高。适合诱导‘玫红细叶青’再生不定芽适宜培养基为MS+6-BA 1.0 mg/L+NAA 0.5 mg/L其不定芽分化率为41.6%,适合‘长叶玫红’不定芽分化的组合是MS+6-BA 1.0 mg/L+NAA 1.0 mg/L其不定芽分化率为33.3%。

The aims were to establish Loropetalum chinense var. rubrum cuitivars efficient regeneration system and lay a foundation for solving the problem of gene engineering breeding. In this paper, the regeneration system of Loropetalum chinense vat. rubrum cuhivars in vitro was studied with leaves as explants. Some factors such as different cuhivars, illumination condition, and hormone concentration and so on were investigated. The results showed the regeneration efficiency of leaves was different. The highest one was in L. chinense vat. rubrum ＇MeihongXiyeqing＇, followed L chinense vat. rubrum ＇Changye Meihong＇ and variegated variation of L chinense vat. rubrum No.2. It could have higher regeneration efficiency when the leaves with the treatment by white light and red light and laid on the medium in adaxial side down, after 70 days, their regeneration frequency were 50% and 25%. The optimal bud regeneration medium from leaves of L chinense var. rubrum ＇Meihong Xiyeqing＇ was MS＋6-BA 1.0 mg/L＋NAA 0.5 rag/L, the induction frequency was 41.6%. The appropriate medium for adventitious bud induction from leaves of L. ehinense vat. rubrum ＇ Changye Meihong＇ was MS＋6-BA1.0 mg/L＋NAA1.0 mg/L, the induction frequency was 33.3%.