蝴蝶兰根内生细菌的分离及可分泌IAA细菌的筛选

The Separation Culture and Screening of IAA-Producing Endophytic Bacteria from the Roots of Phalaenopsis amabilis

为从蝴蝶兰(Phalaenopsis amabilis)根部分离出可分泌吲哚乙酸(indole-3-acetic acid,IAA)的内生细菌,通过探讨蝴蝶兰根部表面消毒的最佳条件,采用分离培养法、生理生化试验和16S rDNA分析,对蝴蝶兰根部内生细菌进行研究。结果表明,NA培养基分离出的6株细菌分别属于短杆菌属(Brevibacterium)、伯克氏菌属(Burkholderia)、Dyella和类芽胞杆菌(Paenibacillus);R2A培养基分离出的20株细菌分别属于伯克氏菌属(Burkholderia)和Dyella;分离获得的26株内生细菌中,具有分泌IAA功能的有23株,占分离总菌数的88.5%。综合来看,蝴蝶兰根中存在可分泌IAA不同种属的内生细菌,为进一步研究内生细菌与蝴蝶兰植物之间的相互作用提供了菌种资源。

In order to separate indole- 3- acetic acid （IAA） producing endophytic bacteria from Phalaenopsis amabilis roots, we investigated the optimal surface sterilization condition of Phalaenopsis amabilis roots, take the separation culture, biological and chemischial characters, and 16S rDNA analysis. The results indicated that, 6 strains isolated by NA medium were Brevibacterium, Burkholderia, Dyella and Paenibacillus. 20 strains isolated by R2A medium were Burkholderia and DyeUa. A total of 23 strains were shown to produce IAA from 26 strains. In general, diverse and abundant communities of IAA-producing endophytic bacteria exist in the roots of Phalaenopsis amabilis which highlight the microbial resources represented by interactions between endophytic bacteria and Phalaenopsis amabilis further.