摘要
为了提取醋酸钙不动杆菌(Actnetobacter calcoaceticus)的PAHs降解酶,利用超声波破碎醋酸钙不动杆菌细胞,对多环芳烃(PAHs)降解酶进行优化提取。在对超声输出功率、工作\间隔时间、菌体稀释倍数以及超声时间进行单因素实验的基础上,利用Box-Behnke中心组合实验设计,响应面法优化得到多环芳烃降解酶的最佳提取工艺为:工作\间隔时间为2 s\2 s、超声功率320W、总超声时间17 min、稀释倍数12.5,在此条件下提取的酶活力为190.48U/g。
Ultrasonic microwave was used in this paper to disrupt the cell of Actnetobacter calcoaceticus WG-6 for PAHs-depredating enzymes. The effect of ultrasound output power, duration of radiation each time, total irradiation time and the cell dilution multiple on the activity of PAHs depredating-enzymes were investigated through the single-factor experiment. The optimum conditions of PAHs depredating-enzymes extraction were determined through box-benhnken center-united design and response surface method were duration of radiation each time 2 s （with a 2 s interval）, ultrasonic power 320 W, total irradiation time 17 min, cell dilution multiple 12.5. The PAHs-depredating enzymes activity was 190.48 U/g under this condition.
出处
《中国农学通报》
CSCD
2014年第17期180-185,共6页
Chinese Agricultural Science Bulletin
基金
国家高技术研究发展计划(863)"半挥发性有机物污染场地物化与生物修复技术"(2013AA06A210)
环保部公益性行业科研专项"盐渍土壤石油-重金属复合污染修复技术及示范"(201109022)
国家国际科技合作专项项目"油泥砂处理中石油吸附菌剂生产技术引进"(2013DFR90540)
关键词
醋酸钙不动杆菌
超声波
多环芳烃降解酶
响应面法
Actnetobacter calcoaceticus
ultrasonication
PAHs-depredating enzymes
response surface method
