大鼠骨骼肌损伤后PMN、MNC及FBC百分率与损伤时间关系

Correlation between Percentages of PMN, MNC, FBC and Wound Age after Skeletal Muscle Injury in Rats

目的：研究大鼠骨骼肌机械性损伤后不同时间点多形核白细胞（polymorphonuclear leucocytes ， PMN）、单个核细胞（mononuclear cells，MNC）及成纤维样细胞（fibroblastic cells，FBC）百分率的变化。方法建立大鼠骨骼肌机械性损伤动物模型，随机分为伤后6h、12h、1d、3d、7d、10d、14d及正常对照组。应用HE染色法和图像分析检测大鼠骨骼肌损伤后不同时间点PMN、MNC及FBC百分率。结果伤后6~12 h，损伤区内可见PMN和MNC浸润，PMN百分率达到峰值；伤后1 d，损伤区内主要以MNC浸润为主，MNC百分率达到峰值，而PMN百分率开始下降；伤后3~7 d，FBC百分率开始逐渐增加，PMN和MNC百分率则逐渐下降；伤后10~14 d，FBC百分率达到峰值。结论大鼠骨骼肌损伤区内PMN、MNC及FBC百分率呈时间规律性变化，有望成为骨骼肌损伤时间推断的参考指标。

Objective To study the percentages of polymorphonuclear leukocytes （PMN）, mononuclear cells （MNC） and fibroblastic cells （FBC） in different posttraumatic intervals after skeletal muscle mechanical injury in rats. Methods The rat model of skeletal muscle mechanical injury was established. The rats were divided into injured groups （6 h, 12 h, 1 d, 3 d, 7 d, 10 d and 14 d after injury ） and control group. The percentages of PMN, MNC and FBC in different posttraumatic intervals after skeletal muscle mechanical injury were assessed with HE staining and image analysis. Results At postinjury 612 h, the percentages of PMN and MNC infiltration appeared in injured sites and that of PMN reached peak. At 1 d, the percentage of MNC infiltration appeared and reached peak, while that of PMN decreased. At 37 d, the percentage of FBC gradually increased, while that of PMN and MNC decreased. At 1014 d, the percentage of FBC reached peak. Conclusion The percentages of PMN, MNC and FBC in injured zones showed timedependent changes, which might be used as reference index for determination of age of skeletal muscle injury.