两种保存液对脱位牙再植术效果影响的实验研究

Experimental research into the influence of two kinds of storage media on the replantation of avulsed tooth

目的:比较常温下脱脂牛奶与汉克斯平衡盐溶液(Hank's balanced salt solution,HBSS)保存脱位牙对再植牙牙根愈合过程的影响。方法:48只6周龄SPF级Wistar雄性大鼠,随机抽取3只为空白对照,直接处死取材拍摄根尖X线片,标本切片组织学观察;余45只随机分为3组,将上颌第一磨牙脱位后分别采用HBSS、脱脂牛奶浸泡或干燥室温保存,30 min后植回牙槽窝,术后1、3、7、14、21 d处死大鼠,拍摄根尖X线片进行图像分析,标本切片进行组织学观察。结果:再植后各时间点牛奶组与HBSS组近中根阴影面积无统计学差异,皆小于干燥组(P<0.01);牛奶组根尖组织炎症反应较轻,根吸收少,根周修复类型与HBSS基本相同。结论:脱脂牛奶与HBSS在常温下均为良好的脱位牙体外保存液,可在普通人群中作为再植牙保存液推广使用。

Objective：To evaluate the effect of skimmed milk and Hank′s balanced salt solution（HBSS）as preservation medium on the root healing and root resorption of the avulsed tooth.Methods：3 out of 48 6-week-old SPF Wistar male rats were picked randomly as blank control,and the rest 45 rats were divided into three groups randomly：HBSS group,milk group and dry group.The bilateral maxillary first molars of rats in the three groups was extracted and replanted back into the sockets after being placed seperately in HB-SS,skimmed milk and in the air for 30 min right after extraction.The rats were sacrificed on day 1,day 3,day 7,day 14 and day 21 after the operation.Then the maxillae were scanned with X-ray and the shadow areas around the mesial roots of maxillary first molars were measured with IPP software.After decalcification,the specimens were made into slices and stained with HE for histological observation.Results：There was no statistically significant difference between milk group and HBSS group regarding the shadow area around mesial roots;the inflammation of root tissue in milk group came to a lighter degree with less resorption,and both the two groups had periodontal healing.Conclusions：Skimmed milk can be recommended as suitable substitute for HBSS and promoted in large-scale.