成石胆汁在胆囊癌致病因素中的作用

Carcinogenicity of bile from cholecystolithiasis patients

目的 探讨人的成石胆汁潜在致癌性 ,阐明胆结石与胆囊癌的内在关系 ,为胆结石的治疗提供科学的依据。方法 通过Ames试验及细胞二阶段转化实验 ,分别检测 18例胆固醇结石患者胆汁的肿瘤启动性 (即致突变性 )和促癌性。结果 Ames实验结果表明 ,胆固醇结石患者的胆汁与正常对照组相比差异无显著意义 ,但可明显增加BALB/c3T3细胞转化灶的形成 (P =0 .0 2 33) ,尤其是无症状胆石组也表现出明显的促癌活性 (P <0 .0 5 )。结论 成石胆汁本身具有潜在的促癌性 ,这一特性在胆囊癌的发病过程中起着重要的作用 ,为胆囊癌的病因学研究提供了直接证据。

Objective To study the carcinogenicity of bile from cholecystolithiasis patients and the relationship between cholecystolithiasis and gallbladder carcinoma (GBC). Methods The bile from 18 cholecystolithiosis patients was examined by Ames test and two-stage cell transformation assay. Results The bile from cholecystolithiosis patients could significantly promote the formation of BALB/c3T3 cell transformation (P=0.023 3). The bile from symptomatic patients was much more active in tumor promotion than that from asymptomatic patients (P<0.05). Conclusion The bile in cholecystolithiasis patients exhibits a tumor-promoter activity, which may account for the high incidence of GBC among cholecystolithiasis patients.