口服雄黄对大鼠脏器中铜、锌、硒含量的影响

Influence of Oral Administration of Realgar on Contents of Copper, Zinc and Selenium in rat Tissues

目的 :探讨大鼠较长时间口服雄黄后体内铜、锌和硒含量的变化。方法 :用不同剂量的雄黄 (5 0 ,15 0 ,45 0mg·kg-1·d-1)给Wistar大鼠持续灌胃 5周 ,测定大鼠主要组织脏器中铜、锌和硒的含量以及肝肾组织中金属硫蛋白的含量 ,并测定实验结束前 5d每只大鼠粪和尿中铜的累积排出量。结果 :口服中低剂量的雄黄对大鼠体内组织中铜、锌和硒水平的影响甚微 ,口服高剂量的雄黄后大鼠心脏中锌含量下降 ,脾脏和骨骼中铜含量升高 ,肾铜水平较对照增加约 2倍。大鼠肝肾组织中金属硫蛋白的含量几乎不受口服雄黄的影响。结论 :肾铜蓄积是大鼠服用高剂量雄黄后体内微量元素变化最为显著的特征 。

Objective:To investigate the changes of copper, zinc and selenium levels in rat tissues after long-term oral administration of Realgar.Methods:Rats were given Realgar with dosage of 50,150,450 mg·kg -1·d -1 for 5 weeks, and the concentrations of copper, zinc and selenium in different rat tissues as well as the contents of metallothionein in rat liver and kidney were determined with atomic absorption and hydride generation-atomic fluorescent technique. The total amount of copper excreted from feces and urine of each rat 5 days before the rat was killed was also measured. Results:No significant changes of levels of copper, zinc and selenium in rat tissues were found after administration of low and middle dosages of Realgar. But higher dosage (450 mg·kg -1·d -1) of Realgar administration could induce a small but significant decrease of zinc concentration in hearts and a increase of copper contents in spleen and tibia, as well as twice more copper concentration of kidney.Conclusion:Copper deposit in kidney was the most significant change found among the trace elements levels in rat tissues, and this might be one of the mechanisms for kidney toxicity of Realgar.