JC病毒T抗原在儿童结直肠幼年性息肉中的表达

The expression of JC virus T-antigen in colorectal juvenile polyps in children

目的探讨JC病毒感染与儿童结直肠幼年性息肉之间的关系。方法应用聚合酶链式反应(PCR)及免疫组化PV-9000法检测35例儿童结直肠幼年性息肉和23例儿童正常结直肠黏膜组织中JC病毒DNA片段及T-Ag蛋白。结果JC病毒DNA片段在儿童结直肠幼年性息肉中的检出率为31.4%(11/35),高于正常结直肠黏膜中的检出率[8.7%(2/23)];T-Ag蛋白在儿童结直肠幼年性息肉中的阳性表达率为22.9%(8/35),在正常结直肠黏膜中无表达;差异有统计学意义(P均<0.05)。在结直肠幼年性息肉患儿的息肉组织中,JC病毒DNA片段检出率和T-Ag蛋白阳性率与患儿年龄、性别及息肉数量、部位、大小无明显关系(P均>0.05)。结论 JC病毒感染可能与儿童结直肠幼年性息肉有关,JC病毒感染可能是儿童结直肠幼年性息肉形成的危险因素之一。

Objective To investigate the relationship between Jamestown Canyon virus （JCV） infection and colorectal juvenile polyps in children. Methods PCR and immunohistochemical staining were performed to detect JCV DNA sequences and localize T-Ag expression in 35 colorectal juvenile polyps and 23 normal colorectal mucosa. Results The positive rate of JCV gene sequences was 31.4%（11 of 35） in the colorectal juvenile polyps and 8.7%（2 of 23） in the normal colorectal mucosa （P0.05）. Conclusions This study suggests a positive association between JCV infection and colorectal juvenile polyps in children, indicating that JCV infection may be a risk factor for colorectal juvenile po-lyps in children.