幽门螺杆菌对克拉霉素耐药的分子机制研究

Molecular mechanism of Helicobacter pylori resistance to clarithromycin.

目的 研究幽门螺杆菌 (Hp)对克拉霉素耐药的分子机制。 方法 用E test进行克拉霉素药敏试验 ,选取治疗前敏感、治疗后耐药的配对菌株及原发耐药Hp菌株进行研究 ;应用随机扩增多态性DNA(RAPD)分析 ,确定治疗前后菌株的同一性 ;用PCR 限制性片段长度多态性 (RFLP)分析探讨克拉霉素耐药机制。结果 9株克拉霉素耐药菌株 2 3SrRNA基因功能区VPCR扩增片段 ,8株被BsaI酶切 ,9株均未被BbsI酶切 ,提示 8株在 2 144位点有A→G突变。结论 上海地区大多数克拉霉素耐药Hp菌株存在 2 3SrRNA基因功能区V 2

Objective To investigate the molecular mechanism of Helicobacter pylori (H.pylori) resistance to clarithromycin. Methods The E test was used to determine clarithromycin resistant strains of H.pylori , and PCR Restriction Fragment Length Polymorphism (RFLP) analysis for 23S rRNA domain V gene mutations. Results Of nine clarithromycin resistant stains of H.pylori , including six primary and three acquired resistant strains, eight were found to have an A to G mutation in 23S rRNA domain V at position 2144. Conclusions The results indicated that the majority (88.8%) of clarithromycin resistant isolates of H.pylori in Shanghai have an A2144G mutation in 23S rRNA domain V.