DLX1基因在人牙齿发育中的表达

Expression of DLX1 Gene in the Human Embryonic Tooth Germ

目的:研究DLX1基因在人牙齿发育中的表达模式,确定DLX1转录子亚型。方法:提取人牙胚总RNA,利用巢式RT-PCR技术克隆DLX1cDNA序列;制备地高辛标记的RNA探针,检测人磨牙发育不同时期DLX1的表达情况。结果:人牙胚中表达的DLX1为转录子亚型1。在表达模式上,11周、14周、17周和19周4个不同发育时期磨牙均有DLX1表达,11周和14周少量表达于牙上皮和间充质,17周依然在牙上皮和间充质中表达,但表达增强,19周表达主要集中在内釉上皮细胞。结论:DLX1在人牙齿发育的各个时期均有表达,表明DLX1可能是人类牙齿发育中的一个重要调节因子。

Objective： To examine the expression pattern and determine the transcript variant of DLX1 gene in the human embryonic tooth germ. Methods： DLX1 cDNA fragment was cloned from the human developing tooth germ by nested RT--PCR. The expression patterns of DLX1 gene at multiple molar developing stages （11W, 14W, 17W and 19W） were examined using biotin--labelled in situ hybridization. Results： DLX1 gene expressed at human tooth germ is transcript variant 1. Weak expression of DLX1 was detected in the dental epithelium and the dental mesenchyme at 11W and 14W, which increased at 17W. At 19W, expression of DLX1 was only restricted in the inner en- amel epithelium. Conclusion： Expression of DLX1 was detected in multiple developing stages of molar, indicating that DLX1 may be a critical regulation factor in human tooth development.