乳腺癌中P53的表达与上皮-间质转化的相关性及其临床意义

Correlation and clinical significance of P53 expression with epithelial-mesenchymal transition in breast cancer

目的:探讨乳腺癌组织中P53的表达和上皮-间质转化(EMT)的相互关系及其临床意义。方法:免疫组织化学法检测63例乳腺癌组织中P53、Twist、Snail、E-Cadherin、N-Cadherin、Vimentin和Fibronectin的表达情况,分析乳腺癌突变型P53蛋白表达与临床病理特征,患者预后和EMT发生之间的关系。结果:P53、Twist和Snail的阳性率以及EMT的发生率分别为44.4%(28/63)、54.0%(34/63)、68.3%(43/63)和41.3%(26/63)。乳腺癌P53表达与组织学分级相关(P<0.05),与其他临床病理特征无关(P>0.05)。P53与Twist、Snail的表达存在相关性,而Twist、Snail和EMT发生相关(P<0.05)。多因素分析显示,淋巴结转移、P53和EMT是乳腺癌患者预后的独立影响因素。结论:P53的表达与乳腺癌组织学分级和EMT的发生相关,是患者预后的独立影响因素,可作为乳腺癌治疗的重要靶点。

Objective：This study aims to investigate the correlation and significance between the expression of P53 and epitheli-al-mesenchymal transition （EMT） in breast cancer. Methods：The expression patterns of P53, Twist, Snail, E-cadherin, N-cadherin, Vi-mentin, and Fibronectin protein were detected via immunohistochemistry in 63 cases with breast carcinoma. The correlation of P53 pro-tein with clinicopathologic features and survival of breast carcinoma, as well as the relationship between the expression of P53 and EMT, was analyzed. Results：The expression rates of P53, Twist, Snail, and EMT are 44.4%（28/63）, 54.0%（34/63）, 68.3%（43/63）, and 41.3%（26/63）, respectively. The P53 protein expression is correlated with tumor grade （P〈0.05） but not with other clinicopatholog-ic features （P〉0.05）. The expression of P53 is also correlated with the expression of Twist and Snail, which are associated with EMT （P〈0.05）. Multivariate survival analysis reveals that lymph node metastasis, P53, and EMT are independent prognostic factors. Conclu-sion：The expression of P53 is correlated with tumor grade and EMT in breast cancer, which can be used as an independent prognostic factor. Therefore, P53 may be an effective target for breast cancer therapy.