叉头框转录因子M1对人结肠癌细胞恶性表型的影响

The fork head box M1 effects on human colon cancer cells malignant phenotype

目的结肠癌的侵袭和转移是结肠癌患者死亡的主要原因。文中拟探讨叉头框蛋白M1(Forkhead box M1,FoxM1)对人结肠癌细胞恶性表型的影响。方法应用实时荧光定量PCR(real time-PCR,RT-PCR)和Western blot方法筛选出高表达细胞株HT-29及低表达细胞株HCT-116,应用RNA干扰技术有效下调FoxM1在HT-29细胞中的表达,同时采用过表达质粒调高FoxM1在HCT-116中的表达,2种细胞株根据不转染、转染空载质粒及转染干扰或过表达FoxM1质粒各分为3组:空白对照组、实验对照组、实验组。分别采用划痕实验和Transwell小室法检测上述转染细胞的细胞增殖、迁移与侵袭能力的变化。结果 RT-PCR及Westen blot结果提示,FoxM1在HT-29细胞中高表达,而在HCT-116细胞中呈低表达。应用PEX-2-FoxM1上调HCT-116细胞中FoxM1表达后,细胞的划痕愈合能力HCT-116实验组[(70.92±1.48)%]较HCT-116实验对照组[(18.43±3.01)%]及HCT-116空白对照组[(16.66±2.63)%]显著增强(P<0.05)。HCT-116实验组Transwell小室穿膜细胞数[(186.0±6.8)个]较HCT-116实验对照组[(42.0±2.0)个]及HCT-116空白对照组[(37.0±2.2)个]均增加(P<0.05)。应用pGPH-sh FoxM1下调HT-29细胞中FoxM1表达后,HT-29实验组细胞的划痕愈合能力[(10.37±3.86)%]较HT-29实验对照组[(39.79±2.17)%]及HT-29空白对照组[(67.36±2.61)%]显著下降(P<0.05)。HT-29实验组Transwell小室穿膜细胞数[(53.0±1.8)个]较较HT-29实验对照组[(95.0±2.2)个]及HT-29空白对照组[(118.0±4.0)个]均减少(P<0.05)。结论FoxM1表达与结肠癌的侵袭、转移等关系密切;FoxM1的siRNA干扰可有效抑制结肠癌细胞的增殖、迁移和侵袭,人为调高结肠癌细胞株中FoxM1表达则促进细胞的增殖、迁移和侵袭。提示FoxM1可能成为抑制结肠癌细胞增殖和转移新的分子靶点。

Objective The invasion and metastasis of colon cancer often leads to treatment failure and mortality in patients.Our research is to investigate the influence of FoxM1 to malignant human colon cancer line.Methods In two human colon cancer lines,the protein and mRNA expression levels of FoxM1 were analyzed with the application of RT-PCR and Western blot,from which high-expressed HT-29 and low-expressed HCT-116 were determined.The expression of FoxM1 was down-regulated by RNA interfering in HT-29 and up-regulated by constructing overexpression transgenic line in HCT-116.The proliferation of the above cells was assayed by healing method; while the metastasis and invasion ability were examined by Transwell chamber assay.Results Two colon cancer lines were selected with high-expression or low-expression of FoxM1 separately named HT-29 and HCT-116.Application of PEX-2-FoxM1 raised after HCT-116 cells express FoxM1,cell scratches in HCT-116 experimetal group（ [70.92 ± 1.48]%） compared with HCT-116 control group（ [16.92 ± 4.05]%） and HCT-116 blank control group（ [16.66 ± 2.63]%） will markedly enhance its capability of healing（ P ＆lt; 0.05）,Transwell Chambers in membrane cells in HCT-116 experimetal group（ 186.0 ± 6.8） compared with HCT-116control group（ 42.0 ± 2.0） and HCT-116 blank control grou（ 37.0 ±2.2） was increased（ P ＆lt; 0.05）.On the other hand,the applied pGPH- shFoxM1 can reduce FoxM1 expression in HT-29 cell,cell scratches healing ability in HT-29 experimetal group（ [10.37 ±3.86]%） compared with HT-29 control group（ [34.63 ± 2.35]%）and HT-29 blank control group（ [67.36 ± 2.61]%） decreased significantly（ P ＆lt; 0.05）,Transwell Chambers in membrane cells in HT-29 experimetal group（ 53.0 ± 1.8） compared with HT-29 control group（ 95.0 ± 2.2） and HT-29 blank control grou（ 118.0 ± 4.0）was also reduced（ P ＆lt; 0.05）.Conclusion The expression of FoxM1 is in close relation to the invasion and metastasis of CRC.The fact that the siRNA interfering FoxM1 could effectively inhibit the proliferation,metastasis and invasion,suggesting FoxM1 could potentially be a new molecular target for inhibiting the proliferation of human colon cancer line.