枳实-白术及其单味药中挥发油成分的对比分析

Comparative Analysis of Volatile Constituents in Citrus aurantium L.-Rhizoma Atractylodis Macrocephalae and its Single Herb

采用水蒸气蒸馏法从枳实、白术及其药对中提取挥发油成分,利用气相色谱-质谱法(GC-MS)分离检测它们,并通过交互移动窗口因子法(AMWFA)对其共有组分进行比较分析,最后通过质谱库结合保留指数、面积积分法进行定性定量分析。枳实、白术及其药对中挥发油分别定性46、45和82个成分,占各自总量的92.51%、91.56%和82.09%。药对与枳实、白术的共有组分分别为38个和37个。药对中挥发油主要成分是:D-苧烯(34.88%)(主要来自枳实);1,2,3,4-四氢-1-丁基异喹啉(22.31%)、大根香叶烯D(4.74%)(主要来自白术);里哪醇(6.17%)(新增)等。实验表明药对种类与含量变化并不是单味药的简单相加。

The volatile constituents in Citrus aurantium L.（ CaL ） -Rhizoma Atractylodis Macrocephalae （ RAM ） and its single herb were extracted by steam distillation , and comparison analysis of these volatile constituents by Gas chromatography-mass spectrometry （ GC-MS ） combined with alternative moving window factor analysis （ AMWFA ） , finally by mass spectrometry library combining retention indices for qualitative and to quantitative by adopting total area in integral method.In total, 46, 45 and 82 volatile chemical components in volatile oil of CaL , RAM and its herbal pair CaL -RAM were separately determined qualitatively , accounting for 92.51%、 91.56% and 82.09% of total contents , respectively.The results showed that there were 38 common volatile constituents in herbal pair and single herb CaL , and 37 common volatile constituents in RAM.The volatile principally components in HP were D -limonene （ 34.88%） （mainly came from the CaL）, 1,2,3,4 -four hydrogen -1 -butyl isoquinoline （22.31%）, Germacrene D（4.74%） （ mainly came from the RAM ） , Farnesol （ 6.17%） （ added new ） , etc.Their relative kinds and amount in HP were not equal to the two single herbs.