HPLC测定金银忍冬叶、花、果实中绿原酸的含量

Determination of Chlorogenic Acid in the Leaves,Flowers and Fruits of Lonicera maackii by High Performance Liquid Chromatography

建立了反相高效液相色谱法测定金银忍冬叶、花和果实中的绿原酸含量的测定方法。利用超声波辅助提取金银忍冬叶、花和果实中的绿原酸,采用采用反相高效液相色谱法测定金银忍冬叶、花和果实中的绿原酸含量。结果表明:在流动相为甲醇(A)∶1.0%的冰醋酸溶液(B)=27∶73,色谱柱为Alltima C18Column(250 mm×4.6 mm,5μm),流动相流速为1.0 mL/min,检测波长为327 nm,柱温为25℃的实验条件下测定金银忍冬叶、花和果实中的绿原酸含量分别为:(31.64±2.21)mg/g,(17.45±1.05)mg/g,(5.14±1.39)mg/g,且宁夏金银忍冬植株不同部位中绿原酸类化合物的含量分布为:叶>花>果实。回收率为99.74%,RSD为0.23%。所建立的方法稳定,操作简单,结果可靠。

Chlorogenic acid in the leaves, flowers and fruits of Lonicera maackii were simultaneously determined by a high performance liquid chromatography method.The samples were separated by a Alltima C18 column using methanol and 1.0% acetic acid as a mixed mobile phase.The detection wavelength was 327 nm.The analytical method displayed a wide linear range in 2-8 μg/mL for the chlorogenic acid.The contents of total chlorogenic acid were （31.64 ±0.07） mg/g and （17.45 ±0.06） mg/g, and（5.14 ±0.27） mg/g in the leaves, flowers and fruits of Lonicera maackii of Ningxia province, respectively.The average recovery was 99.74% and RSD was 0.23%.This method was simple, rapid, accurate and sensitive, and Lonicera maackii of Ningxia was a good source of chlorogenic acids.