Alendronate阻止牙槽骨骨吸收的实验研究

The experiment in rat model with alendronate inhibiting abnormal alveolar bone resorption

目的 评价双膦酸盐Alendronate抑制牙槽骨骨吸收的作用。方法 建立骨质疏松及牙槽骨吸收动物模型 ,建模术后六周 ,给实验组皮下注射Alendronate每周 3次 ,共 6周 ;用血生化指标骨生物力学、骨密度测量、组织形态学等方法进行药效评价。结果 股骨骨密度对照组与用药组相比明显降低 (P <0 .0 1) ;血清碱性磷酸酶对照组与用药组相比明显升高 (P <0 .0 1) ;骨生物力学各项指标实验组与对照组也有明显差异。组织形态学 :实验组牙龈轻度炎症 ,牙槽骨水平吸收与对照组相比明显减少 ;对照组牙龈乳突炎症明显 ,上皮钉突增生 ,炎细胞浸润 ,牙槽骨明显水平吸收。结论 Alendronate治疗骨质疏松有明显的疗效 ,能够增加骨量 ,阻止骨丢失 ;

Objective Osteoporosis and periodontal disease animal models were used to evaluate the anti-resorption effect of alendronate on bone.Methods To establish osteoporosis and alveolar bone resorption animal models.The experimental groups (ovariectomized dental ligature group and simple dental ligature group) were given alendronate in 0.5 mg/kg BW by subcutaneous injection after six weeks of ovariectomy and dental ligature,three times a week for 6 weeks.All the animals were sacrifised 12 weeks after the operation.The blood samples were collected for determination of biochemical indexes.The left femora and jaw bone were processed for histomorphometry.The right femora and mendibles were prepared for determination of bone density and bone biodynamics.Results As compared with the control groups (without alendronate),the values of experimental groups (with alendronate) were all significantly improved on bone density and bone anti-curve power.The alkaline phosphatase,calcium in serum of ovariectomy groups (without alendronate) were obviously increased.In the alendronate group,however,these indexes were close to normal levels.In histomorphometry,there were little inflammation in gingiva and less level alveolar bone resorption in the experimental groups;but there was obvious gingivitis and level alveolar bone resorption in the control groups.Conclusion Alendronate can effectively treat osteoporosis,increase bone volume and prevent bone lose in ovariectomized female rats and inhibit pathological alveolar bone resorption.