大鼠牙周组织中肿瘤坏死因子-α受咬合力增强影响变化的研究

Effect of lost bite force on the expression of TNF-α in the rat periodontium

目的 检测咬合力在正常及增强状态下 ,大鼠牙周细胞TNF -α的动态表达 ,初探TNF -α在牙周组织改建中的分子机理。方法 采用HE染色和免疫组化的方法 ,观察牙周形态变化以及牙周组织中TNF -α蛋白表达。结果 咬合力增强引起牙周膜增宽、牙槽骨形成。牙周细胞中TNF -α表达较正常咬合力时明显增强。结论 咬合力增强 ,促使牙周组织产生TNF -α明显增多 ,诱发了破骨功能 ;同时 ,还激活了成骨功能。本实验从分子水平探讨了牙周组织改建的机理 ;揭示了牙周组织结构与功能在改建中的一致性。

Objective To study the expression of TNF-α in the rat periodontal cells under normal and increased bite force,in order to explain the molecular mechanism of periodontium remodeling.Methods HE staining and immunohistochemistry techniques were applied to study the change in the histologic morphology and the expression of TNF-α protein.Results Under the increased bite force,periodontal ligament width was enlarged and alveolar bone was formed;the expression level of TNF-α increased apparently in periodontal cells.Conclusion Increased bite force induce the expression of TNF-α increased apparently in periodontium.It approached initially the molecular mechanism of periodontium remodeling,suggested that the histologic morphology is closely related to the mechanical condition.