摘要
目的:研究"夹脊"电针对兔退变的腰椎间盘中基质金属蛋白酶13(matrix metalloproteinase-13,MMP-13)和基质金属蛋白酶组织抑制因子1(tissue inhibitor of metalloproteinase-1,TIMP-1)表达的影响,探讨夹脊电针治疗腰椎间盘退行性病变的作用机制。方法:36只清洁级成年新西兰大白兔随机分为正常组、假手术组、模型组和电针组,每组9只。采用克氏针横穿椎体后椎体间加压法复制椎间盘退变模型。电针组针刺L 4、L 5"夹脊"穴28d。各组于第1、28、56天应用Western blot方法检测椎间盘组织中MMP-13蛋白的表达,免疫荧光技术检测椎间盘组织中TIMP-1蛋白的表达。结果:建立模型后,MMP-13蛋白表达增强(P<0.01);电针进行干预后可降低MMP-13的表达(P<0.01)。TIMP-1在荧光激发下,胞质清晰,可见红色荧光,其中各组第1天和正常组、假手术组的第28、56天均呈现强阳性,模型组第56天阳性细胞最少、红色荧光最弱(P<0.01),而电针组在第56天阳性细胞升高(P<0.01)。结论:电针"夹脊"穴可以通过降低椎间盘组织中MMP-13和增强TIMP-1的表达,纠正基质合成与分解代谢失衡,达到防治椎间盘退变的效果。
Objective To observe the effect of electroacupuncture(EA) stimulation of "Jiaji" (EX-B 2) on the expression of matrix metalloproteinase-13 (MMP-13) and tissue inhibitor of metalloproteinase-1 (TIMP-1) proteins in the lumbar disc in rabbits with lumbar intervertebral disc degeneration so as to explore its mechanism in relieving intervertebral disc degeneration. Methods A total of 36 New Zealand rabbits were randomly divided into normal, sham operation (sham), model, and EA groups, with 9 rabbits in each group. The lumbar intervertebral disc degeneration model was established by using a custom-made external loading device to axially compress the lumbar discs (L 4, L 5) for 28 days in reference to Kroeber and colleagues' methods. After modeling, EA stimulation (2 Hz/15 Hz, 1 -2 mA) was applied to bilateral "Jiaji" (EX-B 2) areas for 20 min, once daily for 28 days. The expression levels of MMP-13 and TIMP-1 proteins of the lumbar intervertebral disc (L 4-L 5) tissues were assayed by Western blot and immunoflorescence methods, separately. Results Compared to the normal and sham groups, MMP-13 expression levels at the time-points of day 28 in the model group, on day 28 (pre-EA) in the EA group were significantly up-regulated (P〈0.01); and T[MP-1 expression levels on day 28 in both model and EA groups were significantly decreased (P〈0.01). Following EA treatment, the expression level of MMP-13 was notably lower in the EA group than in the model group, and that of TIMP-1 was remarkably higher in the EA group than in the model group ( P〈0. 01 ). No significant differences were found in the expression levels of MMP-13 and TIMP-1 from day 1 to day 56 in the same one group of both normal and sham groups, and between these two groups (P〉0.05). Conclusion Electroacupuncture at "Jiaji" (EX-B 2) can effectively suppress inter- vertebral disc degeneration induced up-regulation of MMP-13 protein and down-regulation of TIMP-1 protein in the disc tissue in rabbits, which may contribute to its effect in bettering lumbago in clinic.
出处
《针刺研究》
CAS
CSCD
北大核心
2014年第3期192-197,共6页
Acupuncture Research
基金
国家自然科学基金面上项目(No.81173324)
湖北省自然科学基金项目(No.2010CD 034)
湖北省卫生厅年度科研项目[鄂卫函(2010)820号]
关键词
椎间盘退变
电针
夹脊穴
基质金属蛋白酶13
基质金属蛋白酶组织抑制因子1
Intervertebral disc degeneration
Electroacupuncture
Jiaji (EX-B 2)
Matrix metalloproteinase-13
Tissue inhibitor of metalloproteinase-1