针刺“带脉”穴对代谢综合征大鼠体质量、血糖和脂代谢的影响

Effects of Electroacupuncture Stimulation of “Daimai”(GB 26) on Body Weight,Blood Glucose and Blood Lipid Levels in Rats with Metabolism Syndrome

目的:观察针刺"带脉"对代谢综合征大鼠的治疗作用。方法:45只雄性SD大鼠,随机分为空白组15只给基础饲料,造模组30只给予高脂高糖饲料喂养12周。造模成功后,将造模组分为模型组与治疗组,各9只,继续高脂高糖饲料喂养;治疗组取双侧"带脉"穴并给予电针刺激,每次20min,1次/日,连续治疗4周。治疗结束后,测定各组空腹血糖(FBG)、胰岛素(FINS)及血脂含量,血脂包括甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)与低密度脂蛋白胆固醇(LDL-C)。结果:模型组大鼠体质量、腹围、FBG、FINS、稳态模型胰岛素抵抗指数(HOMA-IR)、TG、TC、LDL-C均高于空白组(P<0.01),HDL-C低于空白组(P<0.05);治疗组大鼠体质量、FBG、FINS、HOMA-IR、TG、TC、LDL-C较模型组明显下降(P<0.05,P<0.01),腹围、HDL-C与模型组比较,差异无统计学意义(P>0.05)。结论:针刺"带脉"可以改善代谢综合征大鼠胰岛素抵抗,减轻体质量,调节血脂,降低血糖,起到有效的治疗作用。

Objective To observe the therapeutic effect of electroacupuncture （EA） stimulation of ＂Daimai＂ （GB 26） on metabolic syndrome in rats. Methods SD rats were randomly divided into blank control group （n = 9）, model group （n = 9） and EA group （n = 9）. The metabolic syndrome model was established by feeding the rats with high fat and sugar forage （containing 20% lard, 10% cane sugar, 10% defatted milk powder, 2% cholest, 3% yolk powder and 55% normal forage） for 12 weeks. EA （2 Hz/100 Hz, 1-2 mA） was applied to bilateral ＂Daimai＂ （GB 26） for 20 min, once daily for 4 weeks. Fasting blood glucose（FBG）, serum triglyceride （TG）, total cholesterol （TC）, high density lipoprotein-cholesterol （HDL-C）, lower density lipoprotein-cholesterol （LDL-C） contents were detected using chemical methods,fasting serum insulin （FINS） was determined by using radioimmunoassay. Homeostasis model assessment-index of resistance （HOMA-IR, insulin resistance index = FBG x FINS/22.5） was calculated. Results Compared to the blank control group, the body weight （from the 8th week on） ,abdominal circumference （from the 4th week on）, and FBG, FINS, TG, TO, LDL-C contents as well as HOMA-IR in the model group were significantly increased （P〈0.05, P〈0.01）, while the HDL-C content was considerably lowered after modeling （P〈0.05）. In comparison with the model group, the body weight on the 16^th week, FBG, FINS, HOMA-IR, TG, TC and LDL-C levels were evidently down-regulated in the EA group （P〈0.05, P〈0.01）. No significant differences were found between the model and EA groups in the abdominal circumference and HDL-C content （P〉0.05）. Conclusion Electroacupuncture stimulation of ＂Daimai＂ （GB 26） can reduce insulin resistance, regulate blood lipid, and lower blood glucose in metabolic syndrome rats.